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Open-File Report 2008–1341

Prepared in collaboration with Abraxis, LLC, Delaware Department of Natural Resources and Environmental Control Division of Water Resources Environmental Laboratory, and the University of Delaware

Comparison of Two Cell Lysis Procedures for Recovery of Microcystins in Water Samples from Silver Lake in Dover, Delaware, with Microcystin Producing Cyanobacterial Accumulations

By Keith A. Loftin, Michael T. Meyer, Fernando Rubio, Lisa Kamp, Edythe Humphries, and Ed Whereat

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Abstract

A collaboration was developed between Abraxis, LLC, the State of Delaware Department of Natural Resources and Environmental Control Division of Water Resources Environmental Laboratory, the University of Delaware, and the United States Geological Survey to investigate the efficacy of the QuikLyse™ procedure developed by Abraxis, LLC as an alternative cell-lysis technique suitable for use with an existing liquid chromatography/tandem mass spectrometry research method developed at the United States Geological Survey Organic Geochemistry Research Laboratory to analyze cyanotoxins. A comparison of three sequential freeze/thaw cycles versus QuikLyse™, a proprietary chemical lysis procedure was conducted on four water samples collected from Silver Lake in Dover, Delaware. Results from the Abraxis Microcystins-DM enzyme-linked immunosorbent assay and liquid chromatography/tandem mass spectrometry were tabulated as a function of the cell lysis technique. Stastical comparison of percent relative standard deviations showed no significant difference (α = 0.05) between both cell-lysis techniques when measured by enzyme-linked immunosorbent assay or liquid chromatography/tandem mass spectrometry for three of the four samples.

Version 1.0

Posted November 2008

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Suggested citation:

Loftin, K. A., Meyer, M.T., Rubio, F., Kamp, L., Humphries, E., Whereat, E., 2008, Comparison of two cell lysis procedures for recovery of microcystins in water samples from Silver Lake in Dover, Delaware with microcystin producing cyanobacterial accumulations: USGS Open-File Report 2008–1341, 9 p.



Contents

Abstract

Introduction

Study Design and Method

Results

References Cited


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