Skip Links

USGS - science for a changing world

Open-File Report 2011–1151

A Preliminary Report to the U.S. Coast Guard, Part 2

A Survey of Microbial Community Diversity in Marine Sediments Impacted by Petroleum Hydrocarbons from the Gulf of Mexico and Atlantic Shorelines, Texas to Florida

By John T. Lisle and Sarah H. Stellick

Thumbnail of report cover

Abstract

Microbial community genomic DNA was extracted from sediment samples collected along the Gulf of Mexico and Atlantic coasts from Texas to Florida. Sample sites were identified as being ecologically sensitive and (or) as having high potential of being impacted by Macondo-1 (M-1) well oil from the Deepwater Horizon blowout. The diversity within the microbial communities associated with the collected sediments provides a baseline dataset to which microbial community-diversity data from impacted sites could be compared. To determine the microbial community diversity in the samples, genetic fingerprints were generated and compared. Specific sequences within the community genomic DNA were first amplified using the polymerase chain reaction (PCR) with a primer set that provides possible resolution to the species level. A second nested PCR was performed on the primary PCR products using a primer set on which a GC-clamp was attached to one of the primers. The nested PCR products were separated using denaturing-gradient gel electrophoresis (DGGE) that resolves the nested PCR products based on sequence dissimilarities (or similarities), forming a genomic fingerprint of the microbial diversity within the respective samples. Samples with similar fingerprints were grouped and compared to oil-fingerprint data from the same sites (Rosenbauer and others, 2011). The microbial community fingerprints were generally grouped into sites that had been shown to contain background concentrations of non-Deepwater Horizon oil. However, these groupings also included sites where no oil signature was detected. This report represents some of the first information on naturally occurring microbial communities in sediment from shorelines along the Gulf of Mexico and Atlantic coasts from Texas to Florida.

First posted October 24, 2011

For additional information contact:
John T. Lisle
U.S. Geological Survey
St. Petersburg Coastal and Marine Science Center
600 4th Street South
St. Petersburg, FL 33701

http://coastal.er.usgs.gov/

Part or all of this report is presented in Portable Document Format (PDF); the latest version of Adobe Reader or similar software is required to view it. Download the latest version of Adobe Reader, free of charge.


Suggested citation:

Lisle, J.T., and Stellick, S.H., 2011, A survey of microbial community diversity in marine sediments impacted by petroleum hydrocarbons from the Gulf of Mexico and Atlantic shorelines, Texas to Florida: U.S. Geological Survey Open File-Report 2011–1151, 20 p., available at http://pubs.usgs.gov/of/2011/1151/.



Contents

Abstract

Introduction

Methods

Sampling

Sampling Analyses

Results

Conclusions

Acknowledgments

References Cited


Accessibility FOIA Privacy Policies and Notices

Take Pride in America logo USA.gov logo U.S. Department of the Interior | U.S. Geological Survey
URL: http://pubs.usgs.gov/of/2011/1151/
Page Contact Information: GS Pubs Web Contact
Page Last Modified: Saturday, January 12, 2013, 04:08:14 PM