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SHAKER
BATH STEP
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Materials
Required:
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Fill shaker bath with water to the height of the top of the tube rack and preheat to 95 degrees C. Vortex each Teflon tube until sediment is suspended. |
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Place tubes in shaker bath at shaking frequency 50 and 95 degrees C for 5 hours. *Label and fill each specimen container with 30mL of Milli-Q water using 10mL pipet while shaker bath is operating. Proceed to Centrifuge Step 2. |
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