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Open-File Report 2009–1160

In Cooperation With the Northeast Ohio Regional Sewer District

Results From a Microbial Source-Tracking Study at Villa Angela Beach, Cleveland, Ohio, 2007

By Rebecca N. Bushon, Erin A. Stelzer, and Donald M. Stoeckel

ABSTRACT

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During the 2007 recreational season at Villa Angela Beach in Cleveland, Ohio, scientists with the U.S. Geological Survey (USGS) and the Northeast Ohio Regional Sewer District (NEORSD) found high Escherichia coli (E. coli) concentrations that were not easily explained by results obtained to date in ongoing investigations of recreational water quality at the beach. To help understand the sources behind these elevated E. coli concentrations, the USGS and NEORSD sampled beach-area water for Bacteroides DNA markers. Bacteroides are a group of enteric bacteria that are being used in microbial source tracking, in hope that host-associated DNA markers could be used to indicate potential sources of E. coli in the Villa Angela environment. The USGS Ohio Water Microbiology Laboratory analyzed a total of 13 source samples (sewage and waterfowl feces) and 33 beach-area water and sand samples for three Bacteroides DNA markers. This report lists the results of those analyses, along with environmental conditions at Villa Angela on the dates that samples were collected.

First posted August 7, 2009

For additional information contact:
Director, Ohio Water Science Center
U.S. Geological Survey
6480 Doubletree Avenue
Columbus, OH 43229-1111
http://oh.water.usgs.gov/

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Suggested citation:

Bushon, R.N., Stelzer, E.A., and Stoeckel, D.M., 2009, Results from a microbial source-tracking study at Villa Angela Beach, Cleveland, Ohio, 2007: U.S. Geological Survey Open-File Report 2009–1160, 9 p.


Contents

Abstract

Introduction

Goal and Objectives

Methods

Results

References


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