T.A. Bailey
1983
<div class="abstract-group "><div class="article-section__content en main"><p>Methods were developed for<span> </span><i>in vitro</i><span> </span>screening of candidate aquatic fungicides for efficacy against<span> </span><i>Achlya flagellata, A. racemosa, Saprolegnia hypogyna</i><span> </span>and<span> </span><i>S. megasperma</i>. Agar plugs containing fungal hyphae, removed from the edge of actively growing colonies, were placed in the depressions of spot plates containing 1.0, 10.0 and 100 mg/l of the candidate compounds for 15 or 60 min. After exposure, the plugs were transferred on to filter papers (0.45-μm pore) in a holder, rinsed, and then placed on cornmeal agar medium in tri-petri dishes. The plates were checked for mycelial growth after 48, 96 and 168 h of incubation in a lighted (400–800 μm) environmental control chamber at 20±2°C. Criteria for the acceptance or rejection of candidate aquatic fungicides for further study were based on the antifungal spectrum index (ASI) comparisons between respective compounds and malachite green after 48 h and the concentration level producing complete growth inhibition. Candidate compounds whose ASI was Jess than 50% that of malachite green after 48 h or did not inhibit growth at levels less than 100 mg/l were rejected. This method provides a base from, which<span> </span><i>in vivo</i><span> </span>and definitive test regimens can be developed. Preliminary<span> </span><i>in vitro</i><span> </span>screening of candidate fungicides reduces the need for costly<span> </span><i>in vivo</i><span> </span>tests on compounds that have low antifungal activity.</p></div></div>
application/pdf
10.1111/j.1365-2761.1983.tb00057.x
en
Wiley
Method for in vitro screening of aquatic fungicides
article