V. K. Dawson W.H. Gingerich B. Cheng M.M. Tubergen J.R. Meinertz 1994 <p class="chapter-para">A liquid chromatographic method is described for the determination of sarafloxacin hydrochloride residues in channel catfish (<i>Ictalurus punctatus</i>) fillets. Sarafloxacin was extracted from fillet tissue with acetonitrile–water (1+1). The extract was centrifuged and the supernatant was partitioned with hexane. The aqueous fraction was filtered through a 0.45 μm filter and evaporated to dryness. The sample was redissolved with 20% acetonitrile–methanol (3 + 2) and 80% trifluoroacetic acid (0.1%), centrifuged, and filtered to remove proteins. Samples were analyzed by chromatography with gradient elution on a C₁₈<span>&nbsp;</span>column and with fluorescence detection (excitation at 280 nm and emission above 389 nm). Mean recoveries ranged from 85.4 to 104%, and relative standard deviations ranged from 1.06 to 5.58% in samples spiked at concentrations of 10.0–863.8 ng/g. The method detection limit for sarafloxacin was 1.4 ng/g.</p> application/pdf 10.1093/jaoac/77.4.871 en Oxford Academic Liquid-chromatographic determination of sarafloxacin residues in channel catfish muscle-tissue article