John M. Pearce Barbara J. Pierson Kim T. Scribner W.G. Buchholz 1998 <p>Canada goose (<i>Branta Canadensis</i>) and harlequin duck (<i>Histrionicus histrionicus</i>) DNAs were digested with <i>Sau</i>3AI, and size selected (300-700 bp) fragments were ligated into <i>Bam</i>HI-digested pBluscriptII KS⁺. The enrichment protocol of Ostrander <i>et al</i>.¹ was followed. The resulting libraries were screened using a [ƴ-³²P]ATP end-labelled (CA)₂₀ oligonucleotides as a hybridization probe. Positive clones were sequenced using cycle-sequencing protocols (Epicentre Technologies, Madison, WI) and primers flanking the inserts. PCR primers were designed to amplify the repeat and yield amplification products of ≈100-200 bp. DNA&nbsp; samples were screened for variation at these loci using [ƴ-³²P]ATP end-labelled primers. The products were resolved using 6% denaturing polyacrylamide gels and autoradiography.</p> application/pdf 10.1046/j.1365-2052.1998.00247.x en Wiley Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci article