<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>B.S. Roberson</dc:contributor>
  <dc:contributor>O. W. Dixon</dc:contributor>
  <dc:creator>D. P. Anderson</dc:creator>
  <dc:date>1979</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;Rainbow trout (&lt;/span&gt;&lt;i&gt;Salmo gairdneri&lt;/i&gt;&lt;span&gt;) were exposed to the O-antigen of&amp;nbsp;&lt;/span&gt;&lt;i&gt;Yersinia ruckeri&lt;/i&gt;&lt;span&gt;&amp;nbsp;by various immunization regimens. The passive hemolytic plaque assay was used to show specific splenic plaque-forming cells (PFC) and passive hemagglutination demonstrated humoral antibody titers in fish injected with or immersed in the antigen preparations. Preceding antigen immersion with a 2-min immersion in either a 5.2 or a 2.6% NaCl solution did not affect the numbers of PFC or levels of antibody. Fish held for 2 min in ethyl&amp;nbsp;&lt;/span&gt;&lt;i&gt;m&lt;/i&gt;&lt;span&gt;-aminobenzoate methanesulfonate (MS-222&lt;/span&gt;&lt;sup&gt;®&lt;/sup&gt;&lt;span&gt;) before antigen immersion showed no significant difference from the control fish. Dosage studies showed that the minimal concentration of antigen for induction of PFC by immersion was 5.0 μg/mL.&amp;nbsp;&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1139/f79-092</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Canadian Science Publishing</dc:publisher>
  <dc:title>Plaque-forming cells and humoral antibody in rainbow trout (Salmo gairdneri) induced by immersion in a Yersinia ruckeri O-antigen preparation</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>