J.M. Bertolini
R. C. Cipriano
1988
<div id="9835774" class="article-section-wrapper js-article-section js-content-section " data-section-parent-id="0"><p>Coomassie Brilliant Blue Agar was used to quantify the frequency of the A-layer phenotype in different isolates of<span> </span><i>Aeromonas salmonicida</i>. Hydrophilic, non-clumping isolates of<span> </span><i>A. salmonicida</i><span> </span>consisted predominantly of the A-layer minus phenotype. These bacteria were avirulent by intraperitoneal injection into susceptible brook trout (<i>Salvelinus fontinalis</i>) and could not be reisolated from infected fish. By contrast, hydrophobic, clumping isolates were predominantly of the A-layer positive phenotype, highly virulent in brook trout, and easily recovered from dead or moribund fish. A-layer positive and negative clones of<span> </span><i>A. salmonicida</i><span> </span>were derived by plating bacteria on Coomassie Blue Agar. The plating showed clearly that Coomassie Blue Agar could be used as a highly selective in vitro screening method to reclaim the virulence of certain isolates of<span> </span><i>A. salmonicida</i><span> </span>having a relatively low percentage of A-layer positive phenotypes.</p></div>
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10.7589/0090-3558-24.4.672
en
Wildlife Disease Association
Selection for virulence in the fish pathogen Aeromonas salmonicida, using coomassie brilliant blue agar
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