<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>W. B. Schill</dc:contributor>
  <dc:creator>S. W. Pyle</dc:creator>
  <dc:date>1985</dc:date>
  <dc:description>&lt;p&gt;Techniques are described for the rapid screening of proteinase K-treated bacterial lysates by electroblot and immunoenzymatic detection to assess O-specificity of antigens and antisera. Conditions are outlined which permit the use of a single polyacrylamide gel for both electrotransfer to nitrocellulose and silver staining. Immunodetection of transferred LPS bands was equally sensitive to silver stain when whole cell or O-specific antisera were used. The techniques were utilized to identify at least 4 O-serotypes among sorbitol fermenting isolates of the fish pathogen,&amp;nbsp;&lt;i&gt;Yersinia ruckeri&lt;/i&gt;. Observed variations in the electrophoretic mobilities of lipopolysaccharides from 17 field isolates of&amp;nbsp;&lt;i&gt;Y. ruckeri&lt;/i&gt;&amp;nbsp;were used to accurately predict the O-serotype.&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1016/0022-1759(85)90146-2</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Elsevier</dc:publisher>
  <dc:title>Rapid serological analysis of bacterial lipopolysaccharides by electrotransfer to nitrocellulose</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>