K. Wolf 1962 <p><span>The agent of lymphocystis disease has been propagated under laboratory conditions for more than 2 years. Although several hatchery-propagated centrarchids are susceptible, the bluegill (</span><i>Lepomis macrochirus</i><span>) is the host of choice because it is most easily maintained. At 12.5° the disease was transmitted by implantation and by injection, but there was no evidence of fish-to-fish spread, nor seasonal change in host susceptibility. The infective agent was clearly demonstrated to be filterable (Millipore HA). It retained infectivity in storage at −20° for almost two years, and its viability after desiccation was confirmed. These facts support its postulated viral nature. The virus was glycerol- and ether-sensitive and did not induce formation of giant lymphocystis cells in monolayer cultures of bluegill ovary cells.</span></p> application/pdf 10.1016/0042-6822(62)90011-9 en Elsevier Experimental propagation of lymphocystis disease of fishes article