<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>J.D. Teska</dc:creator>
  <dc:date>1993</dc:date>
  <dc:description>&lt;div class=""&gt;&lt;div class="article-section__content en main"&gt;&lt;p&gt;The development of an assay to quantitate chondroitin AC lyase activity of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Cytophaga columnaris&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;isolates is described. Assay conditions were defined by using&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;C. columnaris&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;originally isolated from channel catfish&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Ictalurus punctatus&lt;/i&gt;, coho salmon&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Oncorhynchus kisutch&lt;/i&gt;, goldfish&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Carassius auratus&lt;/i&gt;, and striped bass&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Morone saxatilis&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;affected with clinical columnaris disease. Supernatant and cellular components of broth cultures exhibited strong activity, and rates of chondroitin sulfate degradation ranged from 20.0 to 81.4 μg/(mL.h) for the cell component and from 35.4 to 83.4 μg/(mL.h) for the supernatant. Degradation rates were calculated by simple linear regression analysis, and most correlations ranged from −0.90 to −1.00. The assay provided results within 2–3 h, and the enzyme is active under a wide range of pH (5–9) and temperature (10–50°C) conditions. The assay can be used as a simple diagnostic aid to differentiate&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;C. columnaris&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;from&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Cytophaga psychrophila&lt;/i&gt;, but more importantly, as a quantitative tool to explore any relationship between specific chondroitin lyase activity and columnaris disease.&lt;/p&gt;&lt;/div&gt;&lt;/div&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1577/1548-8667(1993)005&lt;0259:ATETRK&gt;2.3.CO;2</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Elsevier</dc:publisher>
  <dc:title>Assay to evaluate the reaction kinetics of Chondroitin AC lynase produced by Cytophaga columnaris</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>