<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>R. C. Cipriano</dc:contributor>
  <dc:contributor>N. Gudkovs</dc:contributor>
  <dc:contributor>J Crane . St</dc:contributor>
  <dc:creator>H.K. Byers</dc:creator>
  <dc:date>2001</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;Two&amp;nbsp;&lt;/span&gt;&lt;i&gt;Aeromonas salmonicida&lt;/i&gt;&lt;span&gt;-specific polymerase chain reaction (PCR) tests and 1&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;subsp.&amp;nbsp;&lt;/span&gt;&lt;i&gt;salmonicida&lt;/i&gt;&lt;span&gt;-specific PCR test were used to screen salmonid populations that were either overtly or covertly infected with&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;subsp.&amp;nbsp;&lt;/span&gt;&lt;i&gt;salmonicida&lt;/i&gt;&lt;span&gt;. It was demonstrated that these PCR assays could be used to replace the biochemical testing currently employed to confirm the identity of&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;isolates cultured from infected fish. The AP and PAAS PCR assays were also capable of direct detection of&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;in overtly infected fish, with mucus, gill and kidney samples most likely to yield a positive result. Culture was a more reliable method for the direct detection of&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;in covertly infected salmonids than was the direct PCR testing of tissue samples, with the AP and PAAS PCRs having a lower detection limit (LDL) of approximately 4 x 10&lt;/span&gt;&lt;sup&gt;5&lt;/sup&gt;&lt;span&gt;&amp;nbsp;colony-forming units (CFU) g&lt;/span&gt;&lt;sup&gt;-1&lt;/sup&gt;&lt;span&gt;&amp;nbsp;sample.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.3354/dao049139</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Inter-Research</dc:publisher>
  <dc:title>PCR-based assays for the fish pathogen Aeromonas salmonicida: Further evaluation and validation of three PCR primer sets with infected fish</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>