<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>G. Jeney</dc:contributor>
  <dc:creator>Douglas P. Anderson</dc:creator>
  <dc:date>1991</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;Immunological assays were developed to test for the effects of immunisation with&amp;nbsp;&lt;/span&gt;&lt;i&gt;Aeromonas salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;O antigen bacterin in rainbow trout (&lt;/span&gt;&lt;i&gt;Oncorhynchus mykiss&lt;/i&gt;&lt;span&gt;)&amp;nbsp;&lt;/span&gt;&lt;i&gt;in vitro&lt;/i&gt;&lt;span&gt;&amp;nbsp;and&amp;nbsp;&lt;/span&gt;&lt;i&gt;in vivo&lt;/i&gt;&lt;span&gt;. Trout spleen sections were immunised&amp;nbsp;&lt;/span&gt;&lt;i&gt;in vitro&lt;/i&gt;&lt;span&gt;&amp;nbsp;by incubation in media containing 100, 10, and 1&amp;nbsp;&lt;/span&gt;&lt;i&gt;μ&lt;/i&gt;&lt;span&gt;g ml&lt;/span&gt;&lt;sup&gt;−1&lt;/sup&gt;&lt;span&gt;&amp;nbsp;of the&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;O antigen. After 10 days at 15°C, the individual sections were minced and cell suspensions assayed for the effects of the bacterin on non-specific and specific immune responses. Assays of the cell suspensions showed that the neutrophil oxidative activity, phagocytic capabilities, numbers of plaque-forming cells (PFC) and circulating antibody titres all increased in proportion to the bacterin dosage. Trout immunised&amp;nbsp;&lt;/span&gt;&lt;i&gt;in vivo&lt;/i&gt;&lt;span&gt;&amp;nbsp;with 100&amp;nbsp;&lt;/span&gt;&lt;i&gt;μ&lt;/i&gt;&lt;span&gt;g by injection and by 100&amp;nbsp;&lt;/span&gt;&lt;i&gt;μ&lt;/i&gt;&lt;span&gt;g ml&lt;/span&gt;&lt;sup&gt;−1&lt;/sup&gt;&lt;span&gt;&amp;nbsp;bath with the O antigen, showed similar responses to spleen sections immunised&amp;nbsp;&lt;/span&gt;&lt;i&gt;in vitro&lt;/i&gt;&lt;span&gt;. The appearance of non-specific PFC against unlabelled sheep red blood cells was highest in spleens immunised&amp;nbsp;&lt;/span&gt;&lt;i&gt;in vitro&lt;/i&gt;&lt;span&gt;&amp;nbsp;and in the fish immunised by injection; non-specific PFC rarely occurred in O antigen bathed fish and in control fish. The development of these assays may enable biologists to follow the effects on the non-specific defence mechanisms and the specific immune response in cultivated fish populations after, immunisation with&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;bacterins and thus aid in the development of protective vaccination against furunculosis.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1016/S1050-4648(05)80064-6</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Elsevier</dc:publisher>
  <dc:title>Responses to in vitro and in vivo immunisations with Aeromonas salmonicida O antigen bacterins in rainbow trout (Oncorhynchus mykiss)</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>