<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>E. B. Shotts Jr.</dc:contributor>
  <dc:creator>J.A. Bader</dc:creator>
  <dc:date>1998</dc:date>
  <dc:description>&lt;div class=""&gt;&lt;div class="article-section__content en main"&gt;&lt;p&gt;The nearly complete small subunit 16S ribosomal RNA (rRNA) gene sequence amplified by polymerase chain reaction was determined for&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium psychrophilum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;(formerly&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flexibacter psychrophilus&lt;/i&gt;) by using automated nucleotide sequencing. The sequence was found to be 1,465 base pairs (bp) in length, a size consistent with previously determined sequences for 14 other bacterial species from various taxa, including the yellow-pigmented bacteria. Sequence signatures confirmed that this organism was a member of the bacterial division&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Bacteroides–Flavobacterium.&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;Parsimonious and additive phylogenetic trees were constructed with homology, and pairwise evolutionary distances were used to estimate phylogenetic relationships. Data show that&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;F. psychrophilum, F. columnare&lt;/i&gt;, and&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flexibacter maritimus&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;are closely related, have a common descent, and represent a distinct group within the division&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Bacteroides–Flavobacterium.&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;This group also included other organisms from the genera&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;and&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Cytophaga.&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;Further,&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium aquatile&lt;/i&gt;, the type species for the genus&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium&lt;/i&gt;, was also determined to be a member of this “&lt;i&gt;Flavobacterium–Cytophaga–Flexibacter&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;subcomplex.” This supports previous assertions that the type strain of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;should be changed to a more representative species such as&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Flavobacterium breve&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;&lt;/div&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1577/1548-8667(1998)010&lt;0320:DOPROF&gt;2.0.CO;2</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>American Fisheries Society</dc:publisher>
  <dc:title>Determination of phylogenetic relationships of Flavobacterinium psychrophilum (Flexibacterin psychrophilus), Flavobacterinium columnare (Flexibacterin columnaris), and Flexibacterin maritimus by sequence analysis of 16S ribosomal RNA genes amplified by polymerase chain reaction</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>