E. B. Shotts Jr.
J.A. Bader
1998
<div class="ecommAbs ja publication-tabs publication-tabs-dropdown"><div class="tabs tabs-widget"><div class="tab-content"><div id="mainTabPanel" class="tab tab-pane active" aria-labelledby="showFullText"><div class="hlFld-Abstract"><p class="last">Species-specific polymerase chain reaction (PCR) primers have been developed for the identification of the causative agents of warmwater and marine finrot in fish:<span> </span><i>Flavobacterium columnare</i><span> </span>(<i>Flexibacter columnaris</i>) and<span> </span><i>Flexibacter maritimus.</i><span> </span>Differences in gene sequence in the bacterial small-subunit (16S) ribosomal RNA (rRNA) were used to design the species-specific PCR primers. The previously reported species-specific PCR primers Psy1 and Psy2 for the identification of<span> </span><i>Flavobacterium psychrophilum</i><span> </span>(<i>Flexibacter psychrophila</i>), the causative agent of coldwater finrot, were also used to develop a speciation scheme for all three bacterial finrots. These three primer sets were successful in discriminating among yellow-pigmented bacteria as well as in speciating the three major pathogenic flexibacteria to fish. The primer sets were designed to produce uniquely sized subproducts of 16S rRNA for each species:<span> </span><i>Flavobacterium psychrophilum</i><span> </span>(1,100 base pairs, bp),<span> </span><i>F. columnare</i><span> </span>(800 bp), and<span> </span><i>Flexibacter maritimus</i><span> </span>(400 bp). These primers were shown to correctly speciate field isolates in double-blind experiments (<i>P</i><span> </span>= 0.01).</p></div></div></div></div></div>
application/pdf
10.1577/1548-8667(1998)010<0311:IOFAFS>2.0.CO;2
en
American Fisheries Society
Identification of Flavobacterinium and Flexibacterin species by species-specific polymerase chain reaction primers to the 16S ribosomal RNA gene
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