<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>C. E. Starliper</dc:creator>
  <dc:date>1996</dc:date>
  <dc:description>&lt;p class="abstract_block"&gt;Genetic diversity of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Renibacterium&lt;/i&gt;&lt;i&gt;salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;was evaluated by multilocus enzyme electrophoresis (MEE). Whole cell lysates were prepared for 40 isolates representing 5 groups based on host and geographic area. Each lysate was assessed for activity of 44 enzymes with a pH 6.5 amine-citrate and a pH 8.0 buffer. Genetic variation was scored at 26 loci. Two zones of activity (presumptive loci) were scored each for esterase (EC 3.1.1.1) and glycyl-leucine peptidase (EC 3.4.11.x). There were no monomorphic loci and there was an average of 2.65 electromorphs per locus. There were 21 electrophoretic types. Mean genetic diversity (&lt;i&gt;H&lt;/i&gt;&lt;sub&gt;T&lt;/sub&gt;) was 0.161 and the percentage of this explained by diversity between groups was&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;G&lt;/i&gt;&lt;sub&gt;st&lt;/sub&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;= 8.1%; thus 91.9% of the genetic diversity was due to heterogeneity between individual isolates. The 2 groups with the highest genetic diversity were from chinook&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Oncorhynchus&lt;/i&gt;&lt;i&gt;tshawytscha&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;and coho&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;O&lt;/i&gt;.&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;kisutch&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;salmon, both from the Manistee Weir, Michigan, USA; i.e. 0.270 and 0.298, respectively. The highest genetic diversity for a locus (&lt;i&gt;h&lt;/i&gt;&lt;sub&gt;T&lt;/sub&gt;) was 0.587 for EST-1. At this locus, diversity between groups explained a higher percentage of the total diversity (&lt;i&gt;G&lt;/i&gt;&lt;sub&gt;st&lt;/sub&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;= 36.5%). Other loci with relatively high genetic diversity were succinate dehydrogenase (0.385; EC 1.3.99.1), cytochrome&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;c&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;oxidase (0.273; EC 1.9.3.1) and aconitase (0.311; EC 4.2.1.3). The results of this study indicate relatively low genetic diversity of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;R&lt;/i&gt;.&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;salmoninarum&lt;/i&gt;.&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.3354/dao027207</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Inter-Research</dc:publisher>
  <dc:title>Genetic diversity of North American isolates of Renibacteriumsalmoninarum</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>