R. C. Cipriano
W. B. Schill
J.D. Teska
1992
<div id="9838813" class="article-section-wrapper js-article-section js-content-section " data-section-parent-id="0"><p>Cytochrome oxidase variants of the bacterial fish pathogen,<span> </span><i>Aeromonas salmonicida</i>, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. Protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. Pathogenicity of aberrant isolates to brook trout (<i>Salvelinus fontinalis</i>) was similar to typical cytochrome oxidase-positive isolates. Colorimetric deoxyribonucleic acid (DNA) hybridization in 96-well microplates yielded homology values greater than 82.5% for typical aberrant<span> </span><i>A. salmonicida</i><span> </span>isolates when photobiotinylated DNA for reference<span> </span><i>A. salmonicida</i><span> </span>3.101 was used as a probe. The only variation of these isolates from typical<span> </span><i>A. salmonicida</i><span> </span>was a negative cytochrome oxidase reaction.</p></div>
application/pdf
10.7589/0090-3558-28.4.515
en
Wildlife Disease Association
Molecular and genetic characterization of cytochrome oxidase negative Aeromonas salmonicida isolated from coho salmon (Oncorhynchus kisutch)
article