<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Carol A. Cotant</dc:contributor>
  <dc:creator>Dora R. May Passino</dc:creator>
  <dc:date>1979</dc:date>
  <dc:description>&lt;p&gt;1. Allantoinase, an enzyme in the purine-urea cycle, was found in livers of &lt;i&gt;Salvelinus namaycush&lt;/i&gt; (Osteichthyes: Salmoniformes).&lt;/p&gt;&lt;p&gt;2. The enzyme was active from pH 6.6 to 8.2 at 37°C and from pH 7.4 to 9.0 at 10°C and had an Arrhenius energy was activation of 11.0 kcal/mol and a temperature quotient of 2.0. The &lt;i&gt;K&lt;/i&gt;&lt;sub&gt;&lt;i&gt;m&lt;/i&gt;&lt;/sub&gt; of the enzyme homogenate was 8.4 mM allantoin.&lt;/p&gt;&lt;p&gt;3. The concentrations of inorganic metals at which 50% inhibition occurred during &lt;i&gt;in vitro&lt;/i&gt; exposure were 6.0 mg/l Cu&lt;sup&gt;2+&lt;/sup&gt;, 6.7 mg/l Cd&lt;sup&gt;2+&lt;/sup&gt;, 34 mg/l Hg&lt;sup&gt;2+&lt;/sup&gt; and 52 mg/l Pb&lt;sup&gt;2+&lt;/sup&gt;. The &lt;i&gt;in vitro&lt;/i&gt; sensitivity to PCBs, DDT and DDE and kinetics in the presence of metals were determined.&lt;/p&gt;&lt;p&gt;4. Allantoinase activity was negatively correlated with body length for fish from Lake Michigan but not from Lake Superior or the laboratory.&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1016/0306-4492(79)90102-3</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Elsevier</dc:publisher>
  <dc:title>Allantoinase in lake trout (&lt;i&gt;Salvelinus namaycush&lt;/i&gt;): In vitro effects of PCBs, DDT and metals</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>