L. Cicnjak-Chubbs R.J. Gates M.K. Moore 1994 <p>A selective enrichment procedure, using two new selective media, was developed to isolate <i>Pasteurella multocida</i> from wild birds and environmental samples. These media were developed by testing 15 selective agents with six isolates of <i>P. multocida</i> from wild avian origin and seven other bacteria representing genera frequently found in environmental and avian samples. The resulting media—<i>Pasteurella multocida</i> selective enrichment broth and <i>Pasteurella multocida</i> selective agar—consisted of a blood agar medium at pH 10 containing gentamicin, potassium tellurite, and amphotericin B. Media were tested to determine: 1) selectivity when attempting isolation from pond water and avian carcasses, 2) sensitivity for detection of low numbers of <i>P. multocida</i> from pure and mixed cultures, 3) host range specificity of the media, and 4) performance compared with standard blood agar. With the new selective enrichment procedure, <i>P. multocida</i> was isolated from inoculated (60 organisms/ml) pond water 84% of the time, whereas when standard blood agar was used, the recovery rate was 0%.</p> application/pdf 10.2307/1591956 en American Association of Avian Pathologists A new selective enrichment procedure for isolating Pasteurella multocida from avian and environmental samples article