<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>N. J. Thomas</dc:contributor>
  <dc:contributor>J. P. Dubey</dc:contributor>
  <dc:creator>D. S. Lindsay</dc:creator>
  <dc:date>2000</dc:date>
  <dc:description>&lt;p&gt;&lt;i&gt;Sarcocystis&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;neurona&lt;/i&gt;&lt;span&gt;&amp;nbsp;was isolated from the brain of a juvenile, male southern sea otter (&lt;/span&gt;&lt;i&gt;Enhydra lutris nereis&lt;/i&gt;&lt;span&gt;) suffering from CNS disease. Schizonts and merozoites in tissue sections of the otter’s brain reacted with anti-&lt;/span&gt;&lt;i&gt;S. neurona&lt;/i&gt;&lt;span&gt;&amp;nbsp;antiserum immunohistochemically. Development in cell culture was by endopolyogeny and mature schizonts were first observed at 3 days postinoculation. PCR of merozoite DNA using primer pairs JNB33/JNB54 and restriction enzyme digestion of the 1100 bp product with&amp;nbsp;&lt;/span&gt;&lt;i&gt;Dra&lt;/i&gt;&lt;span&gt;&amp;nbsp;I indicated the organism was&amp;nbsp;&lt;/span&gt;&lt;i&gt;S. neurona&lt;/i&gt;&lt;span&gt;. Four of four interferon-γ gene knockout mice inoculated with merozoites developed&amp;nbsp;&lt;/span&gt;&lt;i&gt;S. neurona&lt;/i&gt;&lt;span&gt;-associated encephalitis. Antibodies to&amp;nbsp;&lt;/span&gt;&lt;i&gt;S.&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;neurona&lt;/i&gt;&lt;span&gt;&amp;nbsp;but not&amp;nbsp;&lt;/span&gt;&lt;i&gt;Sarcocystis falcatula&lt;/i&gt;&lt;span&gt;,&amp;nbsp;&lt;/span&gt;&lt;i&gt;Toxoplasma gondii&lt;/i&gt;&lt;span&gt;, or&amp;nbsp;&lt;/span&gt;&lt;i&gt;Neospora caninum&lt;/i&gt;&lt;span&gt;&amp;nbsp;were present in the serum of inoculated mice. This is the first isolation of&amp;nbsp;&lt;/span&gt;&lt;i&gt;S. neurona&lt;/i&gt;&lt;span&gt;&amp;nbsp;from the brain of a non-equine host.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1016/S0020-7519(00)00034-5</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Elsevier</dc:publisher>
  <dc:title>Biological characterisation of Sarcocystis neurona isolated from a Southern sea otter (Enhydra lutris nereis)</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>