Emily S. Almberg Douglas Smith Sagar Goyal Randall S. Singer L. David Mech 2012 <p><span>Using real-time PCR, we tested 15 wolf (</span><i>Canis lupus</i><span>) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 10</span>⁴<span>&nbsp;50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.</span></p> application/pdf 10.7589/0090-3558-48.2.473 en Wildlife Disease Association Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves article