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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>P. M. Bradley</dc:contributor>
  <dc:creator>C.M. Aelion</dc:creator>
  <dc:date>1991</dc:date>
  <dc:description>&lt;p&gt;In 1975, a leak of 83,000 gallons (314,189 liters) of jet fuel (JP-4) contaminated a shallow water-table aquifer near North Charleston, S.C. Laboratory experiments were conducted with contaminated sediments to assess the aerobic biodegradation potential of the in situ microbial community. Sediments were incubated with&lt;sup&gt; 14&lt;/sup&gt;C-labeled organic compounds, and the evolution of &lt;sup&gt;14&lt;/sup&gt;CO&lt;sub&gt;2&lt;/sub&gt; was measured over time. Gas chromatographic analyses were used to monitor CO&lt;sub&gt;2&lt;/sub&gt; production and O&lt;sub&gt;2&lt;/sub&gt; consumption under aerobic conditions. Results indicated that the microbes from contaminated sediments remained active despite the potentially toxic effects of JP-4. &lt;sup&gt;14&lt;/sup&gt;CO&lt;sub&gt;2&lt;/sub&gt; was measured from [&lt;sup&gt;14&lt;/sup&gt;C]glucose respiration in unamended and nitrate-amended samples after 1 day of incubation. Total [&lt;sup&gt;14&lt;/sup&gt;C]glucose metabolism was greater in 1 mM nitrate-amended than in unamended samples because of increased cellular incorporation of &lt;sup&gt;14&lt;/sup&gt;C label. [&lt;sup&gt;14&lt;/sup&gt;C]benzene and [&lt;sup&gt;14&lt;/sup&gt;C]toluene were not significantly respired after 3 months of incubation. With the addition of 1 mM NO&lt;sub&gt;3&lt;/sub&gt;, CO&lt;sub&gt;2&lt;/sub&gt; production measured by gas chromatographic analysis increased linearly during 2 months of incubation at a rate of 0.099 &lt;span&gt;μ&lt;/span&gt;mol g&lt;sup&gt;-1&lt;/sup&gt; (dry weight) day&lt;sup&gt;-1&lt;/sup&gt; while oxygen concentration decreased at a rate of 0.124 &lt;span&gt;μ&lt;/span&gt;mol g&lt;sup&gt;-1&lt;/sup&gt; (dry weight) day&lt;sup&gt;-1&lt;/sup&gt;. With no added nitrate, CO&lt;sub&gt;2&lt;/sub&gt; production was not different from that in metabolically inhibited control vials. From the examination of selected components of JP-4, the &lt;i&gt;n&lt;/i&gt;-alkane hexane appeared to be degraded as opposed to the branched alkanes of similar molecular weight. The results suggest that the in situ microbial community is active despite the JP-4 jet fuel contamination and that biodegradation may be compound specific. Also, the community is strongly nitrogen limited, and nitrogen additions may be required to significantly enhance hydrocarbon biodegradation.&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1128/aem.57.1.57-63.1991</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>American Society for Microbiology</dc:publisher>
  <dc:title>Aerobic biodegradation potential of subsurface microorganisms from a jet fuel-contaminated aquifer</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>