<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>J.L. Congleton</dc:contributor>
  <dc:creator>D.C. Siegel</dc:creator>
  <dc:date>1997</dc:date>
  <dc:description>&lt;div class="hlFld-Abstract"&gt;&lt;p class="last"&gt;Macrophages isolated from the anterior kidney of juvenile chinook salmon&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Oncorhynchus tshawytscha&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;in 96-well microtiter plates were exposed for 72 h to 0, 10&lt;sup&gt;5&lt;/sup&gt;, or 10&lt;sup&gt;6&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;live or heat-killed&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Renibacterium salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;cells per well or to 0, 0.1, 1.0, or 10 μg/mL of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;R. salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;soluble proteins. After treatment, the bactericidal activity of the macrophages against&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Aeromonas salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;was determined by a colorimetric assay based on the reduction of the tetrazolium dye MTT to formazan by viable bacteria. The MTT assay was modified to allow estimation of the percentage of bacteria killed by reference to a standard curve relating the number of bacteria added to microtiter wells to absorbance by formazan at 600 nm. The live and heatkilled&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;R. salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;treatments significantly (&lt;i&gt;P&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&amp;lt; 0.001) increased killing of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;by chinook salmon macrophages. In each of the five trials, significantly (&lt;i&gt;P&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&amp;lt; 0.05) greater increases in killing occurred after exposure to 10&lt;sup&gt;5&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;R. salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;cells than to 10&lt;sup&gt;6&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;R. salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;cells per well. In contrast, treatment of macrophages with 10 ug/mL&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;R. salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;soluble proteins significantly (&lt;i&gt;P&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;&amp;lt; 0.001) decreased killing of&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;A. salmonicida&lt;/i&gt;, but treatment with lower doses did not. These results show that the bactericidal activity of chinook salmon macrophages is stimulated by exposure to&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;R. salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;cells at lower dose levels but inhibited by exposure to&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;R. salmoninarum&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;cells or soluble proteins at higher dose levels.&lt;/p&gt;&lt;/div&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1577/1548-8667(1997)009&lt;0180:BAOJCS&gt;2.3.CO;2</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Taylor and Francis</dc:publisher>
  <dc:title>Bactericidal activity of juvenile chinook salmon macrophages against Aeromonas salmonicida after exposure to live or heat-killed Renibacterium salmoninarum or to soluble proteins produced by R. salmoninarum</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>