G.S. Wilson E.M. Thurman T. R. Dombrowski 1998 <div class="hlFld-Abstract"><div id="abstractBox"><p class="articleBody_abstractText">Anion-exchange and immunoaffinity particle loaded membranes (PLMs) were investigated as a mechanism for the isolation of charged organic analytes from water. Kinetic properties determined theoretically included dynamic capacity, pressure drop (Δ<i>P</i>), residence and diffusion times (<i>T</i>_r,<span>&nbsp;</span><i>T</i>_d), and total membrane porosity (ε_T). These properties were confirmed through experimental evaluation, and the PLM method showed significant improvement over conventional solid-phase extraction (SPE) and ion-exchange formats. Recoveries of more than 90% were observed for a variety of test compounds at flow rates up to 70 mL/min (equipment-limited maximum flow rate). A fast-flow immunoaffinity column was developed using antibodies (Abs) attached to the PLMs. Reproducible recoveries (88% ± 4%) were observed at flow rates up to 70 mL/min for the antibody (Ab)-loaded PLMs. Findings indicate increased selectivity over anion-exchange PLMs and conventional SPE or ion-exchange methods and rapid Ab−antigen binding rates given the excellent mass-transfer characteristics of the PLMs.</p></div></div> application/pdf 10.1021/ac971081t en ACS Investigation of anion-exchange and immunoaffinity particle-loaded membranes for the isolation of charged organic analytes from water article