<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Tonie E. Rocke</dc:contributor>
  <dc:contributor>Judd M. Aiken</dc:contributor>
  <dc:creator>Judy L. Williamson</dc:creator>
  <dc:date>1999</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;A nested PCR was developed for detection of the&amp;nbsp;&lt;/span&gt;&lt;i&gt;Clostridium botulinum&lt;/i&gt;&lt;span&gt;&amp;nbsp;type C&lt;/span&gt;&lt;sub&gt;1&lt;/sub&gt;&lt;span&gt;&amp;nbsp;toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C&lt;/span&gt;&lt;sub&gt;1&lt;/sub&gt;&lt;span&gt;&amp;nbsp;toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of&amp;nbsp;&lt;/span&gt;&lt;i&gt;C. botulinum&lt;/i&gt;&lt;span&gt;&amp;nbsp;type C in wetland sediments and the sensitivity of the detection assay.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1128/AEM.65.7.3240-3243.1999</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>American Society for Microbiology</dc:publisher>
  <dc:title>In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>