K.L. Warner R.S. Oremland J.C. Murrell I.R. McDonald C.A. Woodall 2001 Strain IMB-1, an aerobic methylotrophic member of the alpha subgroup of the Proteobacteria, can grow with methyl bromide as a sole carbon and energy source. A single cmu gene cluster was identified in IMB-1 that contained six open reading frames: cmuC, cmuA, orf146, paaE, hutI, and partial metF. CmuA from IMB-1 has high sequence homology to the methyltransferase CmuA from Methylobacterium chloromethanicum and Hyphomicrobium chloromethanicum and contains a C-terminal corrinoid-binding motif and an N-terminal methyl-transferase motif. However, cmuB, identified in M. chloromethanicum and H. chloromethanicum, was not detected in IMB-1. application/pdf 10.1128/AEM.67.4.1959-1963.2001 en ASM Identification of methyl halide-utilizing genes in strain IMB-1, a methyl bromide-utilizing bacterium suggests a high degree of conservation of methyl halide-specific genes in gram-negative bacteria article