<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>C.A. Kellogg</dc:contributor>
  <dc:contributor>K.K. Peak</dc:contributor>
  <dc:contributor>E.A. Shinn</dc:contributor>
  <dc:creator>Dale W. Griffin</dc:creator>
  <dc:date>2002</dc:date>
  <dc:description>Aims: A method for the rapid extraction of fungal DNA from small quantities of tissue in a batch-processing format was investigated. Methods and Results: Tissue (&lt; 3.0 mg) was scraped from freshly-grown fungal isolates. The tissue was suspended in buffer AP1 and subjected to seven rounds of freeze/thaw using a crushed dry ice/ethanol bath and a boiling water bath. After a 30 min boiling step, the tissue was quickly ground against the wall of the microfuge tube using a sterile pipette tip. The Qiagen DNeasy Plant Tissue Kit protocol was then used to purify the DNA for PCR/ sequencing applications. Conclusions: The method allowed batch DNA extraction from multiple fungal isolates using a simple yet rapid and reliable assay. Significance and Impact of the Study: Use of this assay will allow researchers to obtain DNA from fungi quickly for use in molecular assays that previously required specialized instrumentation, was time-consuming or was not conducive to batch processing.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1046/j.1472-765x.2002.01071.x</dc:identifier>
  <dc:language>en</dc:language>
  <dc:title>A rapid and efficient assay for extracting DNA from fungi</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>