<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>J. Lisak</dc:contributor>
  <dc:contributor>C. Saltikov</dc:contributor>
  <dc:contributor>P. Basu</dc:contributor>
  <dc:contributor>Ronald S. Oremland</dc:contributor>
  <dc:contributor>J.F. Stolz</dc:contributor>
  <dc:creator>E. Afkar</dc:creator>
  <dc:date>2003</dc:date>
  <dc:description>&lt;p&gt;The respiratory arsenate reductase from the Gram-positive, haloalkaliphile,&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;Bacillus selenitireducens&lt;/i&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;strain MLS10 was purified and characterized. It is a membrane bound heterodimer (150 kDa) composed of two subunits ArrA (110 kDa) and ArrB (34 kDa), with an apparent&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;K&lt;/i&gt;&lt;sub&gt;m&lt;/sub&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;for arsenate of 34 µM and&lt;span&gt;&amp;nbsp;&lt;/span&gt;&lt;i&gt;V&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;of 2.5 µmol min&lt;sup&gt;−1&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;mg&lt;sup&gt;−1&lt;/sup&gt;. Optimal activity occurred at pH 9.5 and 150 g l&lt;sup&gt;−1&lt;/sup&gt;&lt;span&gt;&amp;nbsp;&lt;/span&gt;of NaCl. Metal analysis (inductively coupled plasma mass spectrometry) of the holoenzyme and sequence analysis of the catalytic subunit (ArrA; the gene fr which was cloned and sequenced) indicate it is a member of the DMSO reductase family of molybdoproteins.&lt;/p&gt;&lt;div class="article-metadata-panel clearfix"&gt;&lt;br data-mce-bogus="1"&gt;&lt;/div&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1016/S0378-1097(03)00609-8</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Oxford Academic</dc:publisher>
  <dc:title>The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>