<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>M. W. Rhodes</dc:contributor>
  <dc:contributor>W. K. Vogelbein</dc:contributor>
  <dc:contributor>H. Kator</dc:contributor>
  <dc:contributor>C. A. Ottinger</dc:contributor>
  <dc:creator>David T. Gauthier</dc:creator>
  <dc:date>2003</dc:date>
  <dc:description>&lt;p&gt;Striped&lt;span&gt;&amp;nbsp;&lt;/span&gt;bass&lt;span&gt;&amp;nbsp;&lt;/span&gt;Morone&lt;span&gt;&amp;nbsp;&lt;/span&gt;saxatilis&lt;span&gt;&amp;nbsp;were infected intraperitoneally with approximately 10&lt;/span&gt;&lt;sup&gt;5&lt;/sup&gt;&lt;span&gt;&amp;nbsp;Mycobacterium marinum, M. shottsii sp. nov., or M. gordonae. Infected fish were maintained&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;a flow-through freshwater system at 18 to 21°C, and were examined histologically and bacteriologically at 2, 4, 6, 8, 17, 26, 36 and 45 wk post-infection (p.i.). M. marinum caused acute peritonitis, followed by extensive granuloma development&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;the mesenteries, spleen and anterior kidney. Granulomas&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;these tissues underwent a temporal progression of distinct morphological stages, culminating&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;well-circumscribed lesions surrounded by normal or healing tissue. Mycobacteria were cultured&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;high numbers from splenic tissue at all times p.i. Standard Ziehl-Neelsen staining, however, did not demonstrate acid-fast rods&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;most early inflammatory foci and granulomas. Large numbers of acid-fast rods were present&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;granulomas beginning at 8 wk p.i. Between 26 and 45 wk p.i., reactivation of disease was observed&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;some fish, with disintegration of granulomas, renewed inflammation, and elevated splenic bacterial densities approaching 10&lt;/span&gt;&lt;sup&gt;9&lt;/sup&gt;&lt;span&gt;&amp;nbsp;colony-forming units g&lt;/span&gt;&lt;sup&gt;-1&lt;/sup&gt;&lt;span&gt;. Infection with M. shottsii or M. gordonae did not produce severe pathology. Mild peritonitis was followed by granuloma formation&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;the mesenteries, but, with 1 exception, granulomas were not observed&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;the spleen or anterior kidney. M. shottsii and M. gordonae both established persistent infections&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;the spleen, but were present at densities at least 2 orders of magnitude less than M. marinum at all time points observed. Granulomas&amp;nbsp;&lt;/span&gt;in&lt;span&gt;&amp;nbsp;the mesenteries of M. shottsii- and M. gordonae-infected fish resolved over time, and no reactivation of disease was observed.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.3354/dao054105</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Inter-Research</dc:publisher>
  <dc:title>Experimental mycobacteriosis in striped bass Morone saxatilis</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>