<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>C.O. Ostberg</dc:contributor>
  <dc:contributor>D.R. Clifton</dc:contributor>
  <dc:contributor>J.L. Holloway</dc:contributor>
  <dc:contributor>R. J. Rodriguez</dc:contributor>
  <dc:creator>C. Rasmussen</dc:creator>
  <dc:date>2003</dc:date>
  <dc:description>&lt;p&gt;A marker based on randomly amplified polymorphic DNA (RAPD), OT-38, was discovered that nonlethally discriminates between stream-type and ocean-type populations of chinook salmon Oncorhynchus tshawytscha in the Columbia River basin, including the threatened fall-run (ocean-type) and spring-run (stream-type) Snake River populations. This marker was developed by amplifying chinook salmon genomic DNA with a single RAPD primer, sequencing the termini of the polymorphic products, and designing primer pairs for allele-specific amplification. It was used to assay 18-80 individuals from several wild and hatchery populations differing in year-class, freshwater life history, and location along the Columbia River OT-38 unambiguously distinguished ocean-type from stream-type populations in 93.1% of the chinook salmon sampled.&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1577/1548-8659(2003)132&lt;0131:IOAGMT&gt;2.0.CO;2</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Taylor &amp; Francis</dc:publisher>
  <dc:title>Identification of a genetic marker that discriminates ocean-type and stream-type chinook salmon in the Columbia River basin</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>