John Karl Bohlke Judson W. Harvey Craig R. Tobias 2007 <p><span>We examined the utility of δ</span>¹⁸<span>O</span>₂<span>&nbsp;measurements in estimating gross primary production (P), community respiration (R), and net metabolism (P : R) through diel cycles in a productive agricultural stream located in the midwestern U.S.A. Large diel swings in O</span>₂<span>(±200 µmol L</span>⁻¹<span>) were accompanied by large diel variation in δ</span>¹⁸<span>O</span>₂<span>&nbsp;(±10‰). Simultaneous gas transfer measurements and laboratory‐derived isotopic fractionation factors for O</span>₂<span>during respiration (α</span>_r<span>) were used in conjunction with the diel monitoring of O</span>₂<span>&nbsp;and δ</span>¹⁸<span>O</span>₂<span>to calculate P, R, and P :R using three independent isotope‐based methods. These estimates were compared to each other and against the traditional “open‐channel diel O</span>₂<span>‐change” technique that lacked δ</span>¹⁸<span>O</span>₂<span>. A principal advantage of the δ</span>¹⁸<span>O</span>₂<span>&nbsp;measurements was quantification of diel variation in R, which increased by up to 30% during the day, and the diel pattern in R was variable and not necessarily predictable from assumed temperature effects on R. The P, R, and P :R estimates calculated using the isotope‐based approaches showed high sensitivity to the assumed system fractionation factor (α</span>_r<span>). The optimum modeled ar values (0.986‐0.989) were roughly consistent with the laboratory‐derived values, but larger (i.e., less fractionation) than α</span>_r<span>&nbsp;values typically reported for enzyme‐limited respiration in open water environments. Because of large diel variation in O</span>₂<span>, P :R could not be estimated by directly applying the typical steady‐state solution to the O</span>₂<span>&nbsp;and&nbsp;</span>¹⁸<span>O‐O</span>₂<span>&nbsp;mass balance equations in the absence of gas transfer data. Instead, our results indicate that a modified steady‐state solution (the daily mean value approach) could be used with time‐averaged O</span>₂<span>&nbsp;and δ</span>¹⁸<span>O</span>₂<span>&nbsp;measurements to calculate P :R independent of gas transfer. This approach was applicable under specifically defined, net heterotrophic conditions. The diel cycle of increasing daytime R and decreasing nighttime R was only partially explained by temperature variation, but could be consistent with the diel production/consumption of labile dissolved organic carbon from photosynthesis.</span></p> application/pdf 10.4319/lo.2007.52.4.1439 en ASLO The oxygen-18 isotope approach for measuring aquatic metabolism in high-productivity waters article