Christina A. Kellogg
Julia P. Galkiewicz
2008
<p><span>PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used </span><i>Bacteria</i><span>-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.</span></p>
application/pdf
10.1128/AEM.01303-08
en
ASM journals
Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology
article