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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>J. Huffman</dc:contributor>
  <dc:contributor>Rebecca A. Cole</dc:contributor>
  <dc:contributor>S. Dayanandan</dc:contributor>
  <dc:contributor>V. Tkach</dc:contributor>
  <dc:contributor>J.D. McLaughlin</dc:contributor>
  <dc:creator>L. Bergmame</dc:creator>
  <dc:date>2011</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;Flukes belonging to&amp;nbsp;&lt;/span&gt;&lt;i&gt;&lt;span class="genus-species"&gt;Sphaeridiotrema&lt;/span&gt;&lt;/i&gt;&lt;span&gt;&amp;nbsp;are important parasites of waterfowl, and 2 morphologically similar species&amp;nbsp;&lt;/span&gt;&lt;i&gt;&lt;span class="genus-species"&gt;Sphaeridiotrema globulus&lt;/span&gt;&lt;/i&gt;&lt;span&gt;&amp;nbsp;and&amp;nbsp;&lt;/span&gt;&lt;i&gt;&lt;span class="genus-species"&gt;Sphaeridiotrema pseudoglobulus&lt;/span&gt;&lt;/i&gt;&lt;span&gt;, have been implicated in waterfowl mortality in North America. Cytochrome oxidase I (barcode region) and partial LSU-rDNA sequences from specimens of&amp;nbsp;&lt;/span&gt;&lt;i&gt;&lt;span class="genus-species"&gt;S. globulus&lt;/span&gt;&lt;/i&gt;&lt;span&gt;&amp;nbsp;and&amp;nbsp;&lt;/span&gt;&lt;i&gt;&lt;span class="genus-species"&gt;S. pseudoglobulus&lt;/span&gt;&lt;/i&gt;&lt;span&gt;, obtained from naturally and experimentally infected hosts from New Jersey and Quebec, respectively, confirmed that these species were distinct. Barcode sequences of the 2 species differed at 92 of 590 nucleotide positions (15.6%) and the translated sequences differed by 13 amino acid residues. Partial LSU-rDNA sequences differed at 29 of 1,208 nucleotide positions (2.4%). Additional barcode sequences from specimens collected from waterfowl in Wisconsin and Minnesota and morphometric data obtained from specimens acquired along the north shore of Lake Superior revealed the presence of&amp;nbsp;&lt;/span&gt;&lt;i&gt;&lt;span class="genus-species"&gt;S. pseudoglobulus&lt;/span&gt;&lt;/i&gt;&lt;span&gt;&amp;nbsp;in these areas. Although morphometric data suggested the presence of&amp;nbsp;&lt;/span&gt;&lt;i&gt;&lt;span class="genus-species"&gt;S. globulus&lt;/span&gt;&lt;/i&gt;&lt;span&gt;&amp;nbsp;in the Lake Superior sample, it was not found among the specimens sequenced from Wisconsin or Minnesota.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1645/GE-2370.1</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>American Society of Parasitologists</dc:publisher>
  <dc:title>Sphaeridiotrema globulus and Sphaeridiotrema pseudoglobulus (Digenea): Species differentiation based on mtDNA (Barcode) and partial LSUrDNA sequences</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>