Susan G.W. Kaminsky
Russell J. Rodriguez
Regina S. Redman
Kathleen M. Gough
Merrill Isenor
2010
<div class="capsule__text"><p>FTIR<span> </span>and Raman spectromicroscopy were used to characterize the composition of<span> </span><i>Curvularia protuberata</i><span> </span>hyphae, and to compare a strain isolated from plants inhabiting geothermal soils with a non-geothermal isolate. Thermal<span> </span>IR<span> </span>source images of hyphae have been acquired with a 64 × 64 element<span> </span>focal plane array<span> </span>detector; single point<span> </span>IR spectra<span> </span>have been obtained with<span> </span>synchrotron<span> </span>source light. In some<span> </span><i>C</i><i>. protuberata</i><span> </span>hyphae, we have discovered the spectral signature of crystalline<span> </span>mannitol, a fungal polyol with complex protective roles. With FTIR-FPA imaging, we have determined that the<span> </span>protein<span> </span>content in<span> </span>cells<span> </span>remains fairly constant throughout the length of a hypha, whereas the<span> </span>mannitol<span> </span>is found at discrete, irregular locations. This is the first direct observation of<span> </span>mannitol<span> </span>in intact fungal hyphae. Since the concentration of<span> </span>mannitol<span> </span>in<span> </span>cells<span> </span>varies with respect to position and is not present in all hyphae, this discovery may be related to habitat adaptation, fungal structure and<span> </span>growth<span> </span>stages.</p></div>
application/pdf
10.1039/c0an00534g
en
Royal Society of Chemistry
Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy
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