H. George Ketola
Andrew D. Noyes
William B. Schill
Fred G. Henson
Marc A. Chalupnicki
Dawn E. Dittman
Clifford E. Starliper
2015
<p><span>Diseases of fishes caused by </span><i>Aeromonas</i><span> spp. are common, have broad host ranges and may cause high mortality. Treatments of captive-reared populations using antimicrobials are limited with concerns for bacterial resistance development and environmental dissemination. This study was done to determine whether selected plant-derived essential oils were bactericidal to </span><i>Aeromonas</i><span> spp. Initially, twelve essential oils were evaluated using a disk diffusion assay to an isolate of </span><i>A</i><span>. </span><i>salmonicida</i><span> subsp. </span><i>salmonicida</i><span>, cause of fish furunculosis. The greatest zones of inhibition were obtained with oils of cinnamon </span><i>Cinnamomum cassia</i><span>, oregano </span><i>Origanum vulgare</i><span>, lemongrass </span><i>Cymbopogon citratus</i><span> and thyme </span><i>Thymus vulgaris</i><span>. Minimum bactericidal concentrations (MBC’s) were determined for these four oils, Allimed® (garlic extract, </span><i>Allium sativum</i><span>) and colloidal silver to sixty-nine isolates representing nine </span><i>Aeromonas</i><span> spp. The lowest mean MBCs (0.02–0.04%) were obtained with three different sources of cinnamon oil. MBCs for three sources of oregano and lemongrass oils ranged from 0.14% to 0.30% and 0.10% to 0.65%, respectively, and for two thyme oils were 2.11% and 2.22%. The highest concentration (5%) of Allimed® tested resulted in MBCs to twelve isolates. A concentration of silver greater than 15</span><span> </span><span>mg/L would be required to determine MBCs for all but one isolate.</span></p>
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10.1016/j.jare.2013.12.007
en
Elsevier
An investigation of the bactericidal activity of selected essential oils to Aeromonas spp.
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