George K. Sage Rachel L. DeWilde J. Andres Lopez Sandra L. Talbot Matthew A. Campbell 2014 <p>Blackfishes (Esociformes: Esocidae: <i>Dallia</i>), small fishes with relictual distributions, are unique in being the only primary freshwater fish genus endemic to Beringia. Although the number of species of <i>Dallia</i> is debated, disjunct populations and distinct mitochondrial divisions that predate the end of the last glacial maximum are apparent. We developed sixteen polymorphic microsatellites from the Alaska blackfish (<i>Dallia pectoralis</i>) to study genetic diversity in <i>Dallia</i>. Genotypes from two populations, Denali (n = 31) and Bethel (n = 35), demonstrated the usefulness of the loci for population-level investigation. Observed and expected heterozygosity averaged 18.6 and 19.8 % in Denali and 61.1 and 63.7 % in Bethel. Number of alleles at each locus averaged 3.50 in Denali and 9.63 in Bethel. The observed signature of variability and structuring between populations is consistent with mitochondrial data.</p> application/pdf 10.1007/s12686-013-0091-6 en Springer Development and characterization of 16 polymorphic microsatellite loci for the Alaska blackfish (Esociformes: <i>Dallia pectoralis</i>) article