Cheryl L. Morrison Wendylee Stott Marcus J. Springmann D. Katharine Coykendall 2014 <p><span>A suite of tri- and tetra-nucleotide microsatellite loci were developed for walleye (</span><i class="EmphasisTypeItalic ">Sander vitreus</i><span>) from 454 pyrosequencing data. Eighteen of the 50 primer sets tested amplified consistently in 35 walleye from two lakes on Isle Royale, Lake Superior: Chickenbone Lake and Whittlesey Lake. The loci displayed moderate levels of allelic diversity (average 5.5 alleles/locus) and heterozygosity (average 35.8&nbsp;%). Levels of genetic diversity were sufficient to produce unique multi-locus genotypes and detect phylogeographic structuring as individuals assigned back to their population of origin. Cross-species amplification within </span><i class="EmphasisTypeItalic ">S. canadensis</i><span>(sauger) was successful for 15 loci, and 11 loci were diagnostic to species. The loci characterized here will be useful for detecting fine-scale spatial structuring, resolving the taxonomic status of </span><i class="EmphasisTypeItalic ">Sander</i><span> species and sub-species, and detecting walleye/sauger hybrids.</span></p> application/pdf 10.1007/s12686-014-0275-8 en Springer Development of eighteen microsatellite loci in walleye (<i>Sander vitreus</i>) article