Steven V. Fend David R. Lenat Pilar Rodriguez 2014 <p>Three new species of <i>Lumbriculidae</i> were collected from floodplain seeps and small streams in southeastern North America. Some of these habitats are naturally acidic. <i>Sylphella puccoon</i> <strong>gen. n., sp. n.</strong> has prosoporous male ducts in X-XI, and spermathecae in XII-XIII. Muscular, spherical atrial ampullae and acuminate penial sheaths distinguish this monotypic new genus from other lumbriculid genera having similar arrangements of reproductive organs. <i>Cookidrilus pocosinus</i> <strong>sp. n.</strong> resembles its two subterranean, Palearctic congeners in the arrangement of reproductive organs, but is easily distinguished by the position of the spermathecal pores in front of the chaetae in X-XIII. <i>Stylodrilus coreyi</i> <strong>sp. n.</strong> differs from congeners having simple-pointed chaetae and elongate atria primarily by the structure of the male duct and the large clusters of prostate cells. Streams and wetlands of Southeastern USA have a remarkably high diversity of endemic lumbriculids, and these poorly-known invertebrates should be considered in conservation efforts.</p> application/pdf 10.3897/zookeys.451.7304 en Pensoft Publishers Sylphella puccoon gen. n., sp. n. and two additional new species of aquatic oligochaetes (Lumbriculidae, Clitellata) from poorly-known lotic habitats in North Carolina (USA) article