<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Shaun Baesman</dc:contributor>
  <dc:contributor>Ron Oremland</dc:contributor>
  <dc:creator>Laurence Miller</dc:creator>
  <dc:date>2015</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;We report the first study of stable carbon isotope fractionation during microbial fermentation of acetylene (C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;) in sediments, sediment enrichments, and bacterial cultures. Kinetic isotope effects (KIEs) averaged 3.7&amp;thinsp;&amp;plusmn;&amp;thinsp;0.5&amp;permil; for slurries prepared with sediment collected at an intertidal mudflat in San Francisco Bay and 2.7&amp;thinsp;&amp;plusmn;&amp;thinsp;0.2&amp;permil; for a pure culture of&amp;nbsp;&lt;/span&gt;&lt;i&gt;Pelobacter&lt;/i&gt;&lt;span&gt;&amp;nbsp;sp. isolated from these sediments. A similar KIE of 1.8&amp;thinsp;&amp;plusmn;&amp;thinsp;0.7&amp;permil; was obtained for methanogenic enrichments derived from sediment collected at freshwater Searsville Lake, California. However, C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;&amp;nbsp;uptake by a highly enriched mixed culture (strain SV7) obtained from Searsville Lake sediments resulted in a larger KIE of 9.0&amp;thinsp;&amp;plusmn;&amp;thinsp;0.7&amp;permil;. These are modest KIEs when compared with fractionation observed during oxidation of C&lt;/span&gt;&lt;sub&gt;1&lt;/sub&gt;&lt;span&gt;&amp;nbsp;compounds such as methane and methyl halides but are comparable to results obtained with other C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;compounds. These observations may be useful in distinguishing biologically active processes operating at distant locales in the Solar System where C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;&amp;nbsp;is present. These locales include the surface of Saturn's largest moon Titan and the vaporous water- and hydrocarbon-rich jets emanating from Enceladus.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1089/ast.2015.1355</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Mary Ann Liebert, Inc.</dc:publisher>
  <dc:title>Stable carbon isotope fractionation during bacterial acetylene fermentation: Potential for life detection in hydrocarbon-rich volatiles of icy planet(oid)s</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>