Jodi S. Blum
Marcela A. Ferrero
Sagar M. Utturkar
Steven D. Brown
Barry P. Rosen
Ronald S. Oremland
Daniela Maizel
2015
<p><i>Brevibacterium linens</i><span> AE038-8, isolated from As-contaminated groundwater in Tucumán (Argentina), is highly resistant to arsenic oxyanions, being able to tolerate up to 1 M As(V) and 75 mM As(III) in a complex medium. Strain AE038-8 was also able to reduce As(V) to As(III) when grown in complex medium but paradoxically it could not do this in a defined minimal medium with sodium acetate and ammonium sulfate as carbon and nitrogen sources, respectively. No oxidation of As(III) to As(V) was observed under any conditions. Three copies of the </span><i>ars</i><span> operon comprising arsenic resistance genes were found on </span><i>B. linens</i><span> AE038-8 genome. In addition to the well known </span><i>arsC</i><span>, </span><i>ACR3</i><span> and</span><i>arsR</i><span>, two copies of the </span><i>arsO</i><span> gene of unknown function were detected.</span></p>
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10.1016/j.ibiod.2015.11.022
en
Elsevier Applied Science
Characterization of the extremely arsenic-resistant <i>Brevibacterium linens</i> strain AE038-8 isolated from contaminated groundwater in Tucumán, Argentina
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