J. R. Winton W.N. Batts 1989 <p><span>Infectious hematopoietic necrosis virus (IHNV) was concentrated from water samples by polyethylene glycol (PEG) precipitation, tangential flow filtration (TFF), and by a combination of TFF followed by PEG precipitation of the retentate. Used alone, PEG increased virus titers more than 200-fold, and the efficiency of recovery was as great as 100%. Used alone, TFF concentrated IHNV more than 20-fold, and average recovery was 70%. When the two techniques were combined, 10-L water samples were reduced to about 300 mL by TFF and the virus was precipitated with PEG into a 1 to 2 g pellet; total recovery was as great as 100%. The combined techniques were used to isolate IHNV from water samples taken from a river containing adult sockeye salmon (</span><i>Oncorhynchus nerka</i><span>) and from a hatchery pond containing adult spring chinook salmon (</span><i>O</i><span>. </span><i>tshawytscha</i><span>). The combination of these methods was effective in concentrating and detecting IHNV from water containing only three infectious particles per 10-L sample.</span></p> application/pdf 10.1139/f89-125 en Canadian Science Publishing Concentration of infectious hematopoietic necrosis virus from water samples by tangential flow filtration and polyethylene glycol precipitation article