D.G. Elliott
R.W. Mallett
D. Mulcahy
R.J. Pascho
1987
<p><span>Samples of kidney, spleen, coelomic fluid, and blood from 56 sexually mature coho salmon <i>Oncorhynchus kisutch</i> were examined for infection by <i>Renibacterium salmoninarum</i> by five methods. The overall prevalence (all sample types combined) of <i>R. salmoninarum</i> in the fish was 100% by the enzyme-linked immunosorbent assay, 86% by the combined results of the direct fluorescent antibody and the direct filtration-fluorescent antibody techniques, 39% by culture, 11% by counterimmunoelectrophoresis, and 5% by agarose gel immunodiffusion. There was a significant positive correlation (P < 0.001) between the enzyme-linked immunosorbent assay absorbance levels and the counts by fluorescent antibody techniques for kidney, spleen, and coelomic fluid, and significant positive correlations (P < 0.001) in enzyme-linked immunosorbent assay absorbance levels for all four of the sample types.</span></p>
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10.1577/1548-8659(1987)116<882:COFTFT>2.0.CO;2
en
American Fisheries Society
Comparison of five techniques for the detection of <i>Renibacterium salmoninarum</i> in adult coho salmon.
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