Matthew Rawls
D. Katharine Coykendall
Dionysis I. Foustoukos
Ileana M. Perez-Rodriguez
2016
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<p><span>A novel thermophilic, anaerobic, mixotrophic bacterium, designated strain MAG-PB1</span><sup><span class="jp-sup">T</span></sup><span>, was isolated from a shallow-water hydrothermal vent system in Palaeochori Bay off the coast of the island of Milos, Greece. The cells were Gram-negative, rugose, short rods, approximately 1.0 μm long and 0.5 μm wide. Strain MAG-PB1</span><sup><span class="jp-sup">T </span></sup><span>grew at 30–70 °C (optimum 60 °C), 0–50 g NaCl l</span><sup><span class="jp-sup">− 1</span></sup><span> (optimum 15–20 g l</span><sup><span class="jp-sup">− 1</span></sup><span>) and pH 5.5–8.0 (optimum pH 6.0). Generation time under optimal conditions was 2.5 h. Optimal growth occurred under chemolithoautotrophic conditions with H</span><sub><span class="jp-sub">2</span></sub><span> as the energy source and CO</span><sub><span class="jp-sub">2</span></sub><span> as the carbon source. Fe(III), Mn(IV), arsenate and selenate were used as electron acceptors. Peptone, tryptone, Casamino acids, sucrose, yeast extract, </span><span class="jp-small">d</span><span>-fructose, α-</span><span class="jp-small">d</span><span>-glucose and ( − )-</span><span class="jp-small">d</span><span>-arabinose also served as electron donors. No growth occurred in the presence of lactate or formate. The G+C content of the genomic DNA was 66.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to </span><i><span class="jp-italic">Deferrisoma camini</span></i><span>, the first species of a recently described genus in the </span><i><span class="jp-italic">Deltaproteobacteria</span></i><span>. Based on the 16S rRNA gene phylogenetic analysis and on physiological, biochemical and structural characteristics, the strain was found to represent a novel species, for which the name </span><i><span class="jp-italic">Deferrisoma palaeochoriense</span></i><span> sp. nov. is proposed. The type strain is MAG-PB1</span><sup><span class="jp-sup">T</span></sup><span> ( = JCM 30394</span><sup><span class="jp-sup">T</span></sup><span> = DSM 29363</span><sup><span class="jp-sup">T</span></sup><span>).</span> </p>
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10.1099/ijsem.0.000798
en
SGM
<i>Deferrisoma paleochoriense</i> sp. nov., a thermophilic, iron(III)-reducing bacterium from a shallow-water hydrothermal vent in the Mediterranean Sea
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