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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Robert Benjamin Snipes</dc:contributor>
  <dc:contributor>Ann M. Thompson</dc:contributor>
  <dc:contributor>Donald W. Schloesser</dc:contributor>
  <dc:creator>David M. Malakauskas</dc:creator>
  <dc:date>2016</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;We used PCR to screen pooled individuals of&amp;nbsp;&lt;/span&gt;&lt;i&gt;Manayunkia speciosa&lt;/i&gt;&lt;span&gt;&amp;nbsp;from western Lake Erie, Michigan, USA for myxosporean parasites. Amplicons from positive PCRs were sequenced and showed a&amp;nbsp;&lt;/span&gt;&lt;i&gt;Ceratonova&lt;/i&gt;&lt;span&gt;&amp;nbsp;species in an estimated 1.1% (95% CI = 0.46%, 1.8%) of&amp;nbsp;&lt;/span&gt;&lt;i&gt;M&lt;/i&gt;&lt;span&gt;.&amp;nbsp;&lt;/span&gt;&lt;i&gt;speciosa&lt;/i&gt;&lt;span&gt;&amp;nbsp;individuals. We sequenced 18S, ITS1, 5.8S, ITS2 and most of the 28S rDNA regions of this&amp;nbsp;&lt;/span&gt;&lt;i&gt;Ceratonova&lt;/i&gt;&lt;span&gt;&amp;nbsp;sp., and part of the protein-coding EF2 gene. Phylogenetic analyses of ribosomal and EF2 sequences showed the Lake Erie&amp;nbsp;&lt;/span&gt;&lt;i&gt;Ceratonova&lt;/i&gt;&lt;span&gt;&amp;nbsp;sp. is most similar to, but genetically distinct from,&amp;nbsp;&lt;/span&gt;&lt;i&gt;Ceratonova shasta&lt;/i&gt;&lt;span&gt;. Marked interspecific polymorphism in all genes examined, including the ITS barcoding genes, along with geographic location suggests this is an undescribed&amp;nbsp;&lt;/span&gt;&lt;i&gt;Ceratonova&amp;nbsp;&lt;/i&gt;&lt;span&gt;species. COI sequences showed&amp;nbsp;&lt;/span&gt;&lt;i&gt;M&lt;/i&gt;&lt;span&gt;.&amp;nbsp;&lt;/span&gt;&lt;i&gt;speciosa&lt;/i&gt;&lt;span&gt;&amp;nbsp;individuals in Michigan and California are the same species. These findings represent a third parasite in the genus&amp;nbsp;&lt;/span&gt;&lt;i&gt;Ceratonova&lt;/i&gt;&lt;span&gt;potentially hosted by&amp;nbsp;&lt;/span&gt;&lt;i&gt;M&lt;/i&gt;&lt;span&gt;.&amp;nbsp;&lt;/span&gt;&lt;i&gt;speciosa&lt;/i&gt;&lt;span&gt;.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1016/j.jip.2016.05.001</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Society for Invertebrate Pathology</dc:publisher>
  <dc:title>Molecular evidence of undescribed &lt;i&gt;Ceratonova&lt;/i&gt; sp. (Cnidaria: Myxosporea) in the freshwater polychaete, &lt;i&gt;Manayunkia speciosa&lt;/i&gt;, from western Lake Erie</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>