Chienjin Huang
Shih-Ling Hsuan
Zeng-Weng Chen
Wei-Cheng Lee
Cheng-I Liu
James R. Winton
Maw-Sheng Chien
Chih-Ming Liao
2006
<p><span>Three short fragments of recombinant subunit </span><i>Pasteurella multocida</i><span> toxin (rsPMT) were constructed for evaluation as candidate vaccines against progressive atrophic rhinitis (PAR) of swine. PMT-specific antibody secreting cells and evidence of cellular immunity were detected in rsPMT-immunized pigs following authentic PMT challenge or homologous antigen booster. Piglets immunized with rsPMT fragments containing either the N-terminal or the C-terminal portions of PMT developed high titers of neutralizing antibodies. Pregnant sows immunized with rsPMT had higher levels of maternal antibodies in their colostrum than did those immunized with a conventional PAR-toxoid vaccine. Offspring from rsPMT vaccinated sows had better survival after challenge with a five-fold lethal dose of authentic PMT and had better growth performance after challenge with a sublethal dose of toxin. Our findings indicate these non-toxic rsPMT proteins are attractive candidates for development of a subunit vaccine against PAR in pigs.</span></p>
application/pdf
10.1016/j.vaccine.2005.07.079
en
Elsevier
Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs
article