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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Donald E. Tillitt</dc:contributor>
  <dc:contributor>Janusz Z. Byczkowski</dc:contributor>
  <dc:contributor>G. Allen Burton Jr.</dc:contributor>
  <dc:contributor>Stephen R. Channel</dc:contributor>
  <dc:contributor>Joanne M. Drerup</dc:contributor>
  <dc:contributor>Carlyle D. Flemming</dc:contributor>
  <dc:contributor>Jeffrey W. Fisher</dc:contributor>
  <dc:creator>Kyung O. Yu</dc:creator>
  <dc:date>1996</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;The rat hepatoma cell line, H4IIE, serves as a useful tool to assess potential biological effects such as induction of cytochrome P4501A1 expression. The objectives of this study were twofold: to investigate the kinetic time course and dosimetry of PCB77 in rat hepatoma cells dosed with PCB77 and in liver of rats given ip doses of PCB77, and to compare&lt;/span&gt;&lt;i&gt;in vitro&lt;/i&gt;&lt;span&gt;and&lt;/span&gt;&lt;i&gt;in vivo&lt;/i&gt;&lt;span&gt;P4501A1 enzyme induction responses. For the 4-day time–course study, H4IIE cells were exposed with two doses of [&lt;/span&gt;&lt;sup&gt;14&lt;/sup&gt;&lt;span&gt;C]PCB77 (0.9 and 3 μg/plate) and harvested at 15 and 30 min, 1, 2, 4, 8, and 12 hr, and 1, 2, 3, and 4 days. PCB77-derived radioactivity was detected in the cells as early as 15 min postdosing. For the dose–response study, the cells were dosed with various concentrations of PCB77 (0.00316–5.37 μg/plate) and harvested on Day 3 since ethoxyresorufin&lt;/span&gt;&lt;i&gt;O&lt;/i&gt;&lt;span&gt;-deethylase (EROD) activity&lt;/span&gt;&lt;i&gt;in vitro&lt;/i&gt;&lt;span&gt;reached its maximum on the third day postdosing. Time–course and dose–response studies revealed that only 1–3% of the total delivered dose was found in the cells, with the remainder in the media and adhering to the culture plates. For the dose–response study&lt;/span&gt;&lt;i&gt;in vivo,&lt;/i&gt;&lt;span&gt;male Fischer rats were dosed with a single ip injection of various concentrations of PCB77 (0.1–50 mg/kg body wt) and euthanized on Day 3. PCB77-derived radioactivity and EROD induction&lt;/span&gt;&lt;i&gt;in vivo&lt;/i&gt;&lt;span&gt;were measured. When EROD activity and PCB77-derived radioactivity in the rat hepatoma cells and in the rat liver were compared on an equivalent weight basis, there was a significant correlation (&lt;/span&gt;&lt;i&gt;r&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt;&lt;span&gt;= 0.985) between them. Prior to this study, no information on quantitative dosimetry and EROD activities of PCB77 has been reported to validate the&lt;/span&gt;&lt;i&gt;in vitro&lt;/i&gt;&lt;span&gt;assay with&lt;/span&gt;&lt;i&gt;in vivo&lt;/i&gt;&lt;span&gt;data.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1006/taap.1996.0309</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>Elsevier</dc:publisher>
  <dc:title>In vivo/in vitro comparison of pharmacokinetics and pharmacodynamics of 3,3',4,4'-tetrachlorobiphenyl (PCB77)</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>