R. J. Rodriguez S. Freeman 1995 <p><span>A collection of 39 isolates of </span><i>Colletotrichum acutatum, C. fragariae</i><span> and </span><i>C. gloeosporioides</i><span>, which cause anthracnose on strawberry, was grouped into species based on the arbitrarily primed polymerase chain reaction (ap-PCR). All isolates used had previously been identified according to classical taxonomic morphology. Ap-PCR amplification of genomic DNA using four different primers allowed for reliable differentiation between isolates of </span><i>C. acutatum, C. fragariae</i><span> and two genotypes of </span><i>C. gloeosporioides</i><span>. Fifteen of the 18 </span><i>C. acutatum</i><span> isolates were very similar, although three isolates which produced a red pigment had distinctly different banding patterns. Nearly identical banding patterns were observed for all nine isolates of </span><i>C. fragariae</i><span>. The 12 </span><i>C. gloeosporioides</i><span> isolates were more diverse and two separate genotypes, Cgl-1 (six isolates) and Cgl-2 (five isolates) were distinguished by ap-PCR. An additional isolate did not conform to either the Cgl-1 or Cgl-2 genotypes. The utility of ap-PCR compared with other molecular techniques for reliable identification of </span><i>Colletotrichum</i><span> isolates pathogenic on strawberry is discussed.</span></p> application/pdf 10.1016/S0953-7562(09)80653-9 en Elsevier Differentiation of Colletotrichum species responsible for anthracnose of strawberry by arbitrarily primed PCR article