Teresa L. Gilbert Chienjin Huang Marsha L. Landolt Patrick J. O’Hara James R. Winton Maw-Sheng Chien 1992 <p><span>The complete sequence coding for the 57-kDa major soluble antigen of the salmonid fish pathogen, </span><i>Renibacterium salmoninarum</i><span>, was determined. The gene contained an opening reading frame of 1671 nucleotides coding for a protein of 557 amino acids with a calculated </span><i>M</i>_r<span> value of 57190. The first 26 amino acids constituted a signal peptide. The deduced sequence for amino acid residues 27–61 was in agreement with the 35 N-terminal amino acid residues determined by microsequencing, suggesting the protein in synthesized as a 557-amino acid precursor and processed to produce a mature protein of </span><i>M</i>_r<span> 54505. Two regions of the protein contained imperfect direct repeats. The first region contained two copies of an 81-residue repeat, the second contained five copies of an unrelated 25-residue repeat. Also, a perfect inverted repeat (including three in-frame UAA stop codons) was observed at the carboxyl-terminus of the gene.</span></p> application/pdf 10.1111/j.1574-6968.1992.tb05427.x en Wiley Molecular cloning and sequence analysis of the gene coding for the 57kDa soluble antigen of the salmonid fish pathogen Renibacterium salmoninarum article