<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:contributor>Ronald S. Oremland</dc:contributor>
  <dc:contributor>Tong Liu</dc:contributor>
  <dc:contributor>Abigail A Landers</dc:contributor>
  <dc:contributor>Shaun Baesman</dc:contributor>
  <dc:contributor>Lisa Alvarez-Cohen</dc:contributor>
  <dc:creator>Xinwei Mao</dc:creator>
  <dc:date>2017</dc:date>
  <dc:description>&lt;p&gt;&lt;span&gt;Acetylene (C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;) can be generated in contaminated groundwater sites as a consequence of chemical degradation of trichloroethene (TCE) by in situ minerals, and C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt; is known to inhibit bacterial dechlorination. In this study, we show that while high C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt; (1.3 mM) concentrations reversibly inhibit reductive dechlorination of TCE by &lt;/span&gt;&lt;i&gt;Dehalococcoides mccartyi&lt;/i&gt;&lt;span&gt; isolates as well as enrichment cultures containing &lt;/span&gt;&lt;i&gt;D. mccartyi&lt;/i&gt;&lt;span&gt; sp., low C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt; (0.4 mM) concentrations do not inhibit growth or metabolism of &lt;/span&gt;&lt;i&gt;D. mccartyi&lt;/i&gt;&lt;span&gt;. Cocultures of &lt;/span&gt;&lt;i&gt;Pelobacter&lt;/i&gt;&lt;span&gt; SFB93, a C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;-fermenting bacterium, with &lt;/span&gt;&lt;i&gt;D. mccartyi&lt;/i&gt;&lt;span&gt; strain 195 or with &lt;/span&gt;&lt;i&gt;D. mccartyi&lt;/i&gt;&lt;span&gt; strain BAV1 were actively sustained by providing acetylene as the electron donor and carbon source while TCE or &lt;/span&gt;&lt;i&gt;cis&lt;/i&gt;&lt;span&gt;-DCE served as the electron acceptor. Inhibition by acetylene of reductive dechlorination and methanogenesis in the enrichment culture ANAS was observed, and the inhibition was removed by adding &lt;/span&gt;&lt;i&gt;Pelobacter&lt;/i&gt;&lt;span&gt; SFB93 into the consortium. Transcriptomic analysis of &lt;/span&gt;&lt;i&gt;D. mccartyi&lt;/i&gt;&lt;span&gt; strain 195 showed genes encoding for reductive dehalogenases (e.g., &lt;/span&gt;&lt;i&gt;tceA&lt;/i&gt;&lt;span&gt;) were not affected during the C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;-inhibition, while genes encoding for ATP synthase, biosynthesis, and Hym hydrogenase were down-regulated during C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt; inhibition, consistent with the physiological observation of lower cell yields and reduced dechlorination rates in strain 195. These results will help facilitate the optimization of TCE-bioremediation at contaminated sites containing both TCE and C&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;H&lt;/span&gt;&lt;sub&gt;2&lt;/sub&gt;&lt;span&gt;.&lt;/span&gt;&lt;/p&gt;</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>10.1021/acs.est.6b05770</dc:identifier>
  <dc:language>en</dc:language>
  <dc:publisher>American Chemical Society</dc:publisher>
  <dc:title>Acetylene fuels TCE reductive dechlorination by defined &lt;i&gt;Dehalococcoides&lt;/i&gt;/&lt;i&gt;Pelobacter&lt;/i&gt; consortia</dc:title>
  <dc:type>article</dc:type>
</oai_dc:dc>